|Year : 2018 | Volume
| Issue : 5 | Page : 579-583
Influence of different reference databases on categorization of bone mineral density: A study on rural postmenopausal women from Southern India
Kripa Elizabeth Cherian, Nitin Kapoor, Hesarghatta Shyamasunder Asha, Nihal Thomas, Thomas Vizhalil Paul
Department of Endocrinology, Christian Medical College, Vellore, Tamil Nadu, India
|Date of Web Publication||18-Sep-2018|
Thomas Vizhalil Paul
Department of Endocrinology, Christian Medical College, Vellore - 632 004, Tamil Nadu
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background and Objectives: Currently available DXA (Dual energy X-ray Absorptiometry) scanners utilise bone mineral density (BMD) of Caucasian population to calculate T scores and categorise BMD. We studied the influence of various databases on classification of BMD in south-Indian postmenopausal women aged above 50 years. Methodology: This was a cross-sectional study. Hologic DXA scanner was used to estimate BMD at lumbar spine (LS) and femoral neck (FN). T scores of ≤-2.5, -2.4 to -1, -0.9 to +1 were diagnostic of osteoporosis, osteopenia and normal respectively. Three reference databases(Italian, Korean and north Indian) were used to recalculate T scores. The agreement (K=kappa) between manufacturer provided database and the other databases was studied. The impact of different databases in diagnosing osteoporosis in subjects with FN fracture was assessed. Results: A total of 1956 postmenopausal women with mean (SD) age of 62 (4.3) years and 211 femoral neck(FN) fracture subjects with mean(SD) age of 68 (7.2) years were recruited. In subjects with fracture, osteoporosis at FN was found in 72% with Caucasian, 88% with North Indian, 56% with Italian, and 45% with Korean database. On comparing manufacturer provided database with the other population-specific reference, there was perfect agreement with north Indian (κ = 0.81 [FN], κ = 0.82 [LS]) and good agreement with the Italian database (κ = 0.78 [FN], κ = 0.74 [LS]). Conclusion: North-Indian database identified most of the participants with FN fracture as having osteoporosis and had perfect agreement with the manufacturer's database. Follow up studies will further validate the impact of utilizing this database in clinical practice.
Keywords: Osteoporosis, population-specific database, fracture
|How to cite this article:|
Cherian KE, Kapoor N, Asha HS, Thomas N, Paul TV. Influence of different reference databases on categorization of bone mineral density: A study on rural postmenopausal women from Southern India. Indian J Endocr Metab 2018;22:579-83
|How to cite this URL:|
Cherian KE, Kapoor N, Asha HS, Thomas N, Paul TV. Influence of different reference databases on categorization of bone mineral density: A study on rural postmenopausal women from Southern India. Indian J Endocr Metab [serial online] 2018 [cited 2019 Apr 18];22:579-83. Available from: http://www.ijem.in/text.asp?2018/22/5/579/241499
| Introduction|| |
Osteoporosis, a common metabolic bone disease is a significant cause of morbidity in postmenopausal women and elderly men. In India, a significant proportion of the population >50 years has osteoporosis and fragility fractures. Fragility fractures, especially of the femoral neck (FN) has been associated with mortality of about 10%–20%., Widely prevalent nutritional calcium and Vitamin D deficiency in developing countries like India also further contribute adversely to bone health. The gold standard for the diagnosis of osteoporosis is bone mineral density (BMD) measurement at lumbar spine, FN, and forearm by a dual-energy X-ray absorptiometry scan (DXA) and expressed as T-scores which is the number of standard deviations (SDs) of the patient's measured BMD from a reference peak BMD (PBMD) (mean BMD of gender-matched young adults). A T score ≤−2.5 at any site is considered as diagnosis of osteoporosis and a score between −1 and −2.5 as osteopenia.
The DXA Manufacturers (Hologic, GE Lunar, Norland) have incorporated Caucasian-based reference PBMD to calculate T scores. However, there seem to be wide variations in the attainment of PBMD based on ethnicity differences, genetic and environmental influences. Thus, the use of different reference databases may influence the diagnosis of osteoporosis, leading to either underdiagnosis or overdiagnosis of low BMD. There have been studies from few countries which estimated the PBMD which was specific to a particular ethnicity and utilized them as a reference population to derive T scores for the diagnosis of osteoporosis. Few studies have also looked at the differences in diagnosis of osteoporosis when manufacturer based versus ethnicity-specific reference BMD were used., Shetty et al. looked at the impact of using an Indian population-based database obtained from four cities (Hyderabad, Delhi, Lucknow, and Mumbai) versus Hologic database in classifying postmenopausal women into various categories (normal, osteopenia, and osteoporosis) and found that although agreement between them was good, a significant proportion of subjects with FN fracture were classified as osteopenia rather than osteoporosis when Indian reference database was used. Recently, Marwaha et al. published an age-specific BMD reference range for the Indian population using DXA scan in a healthy cohort of 2034 Indian women aged 18–85 years. There are other country-specific databases which could have either a positive or negative impact on the diagnosis of low BMD. Their influence in the diagnosis of osteoporosis in the Indian context has not been studied. Hence, we attempted to study the influence of three databases (North Indian, Italian, and Korean) versus manufacturer database on categorization of BMD of south Indian postmenopausal women into normal, osteopenia and osteoporosis at lumbar spine and FN and also studied the agreement between three databases and manufacturer database in the diagnosis of osteoporosis. We also looked at the impact of utilizing these databases in diagnosing osteoporosis in a cohort of postmenopausal women with recent FN fracture.
| Methodology|| |
This was a cross-sectional study in healthy ambulatory rural south Indian women conducted over a period of 18 months at a tertiary care center (January 2016 to June 2017). The study was approved by the Institutional Review Board. Consecutive ambulant postmenopausal women (>50 years of age) from rural south India were recruited into the study. We also included a cohort of hospital based patients (admitted during this period) who had sustained hip fracture (n = 211) following a trivial fall (<2 weeks duration). Weight in kilogram was recorded using an electronic scale and standing height in centimeter was measured with a stadiometer. The formula of weight (kg)/height in meter  was used to calculate body mass index (BMI). Blood samples were collected in fasting state for the measurement of serum calcium (n = 8.3–10.4 mg/dL), phosphorus (n = 2.5–4.5 mg/dL), albumin (n = 3.5–5.0 g/dL), creatinine (n = 0.6–1.4 mg/dl), and 25-hydroxyvitamin D (n = 30–75 ng/mL). BMD at the lumbar spine and FN was assessed with Hologic QDR 4500 Discovery A DXA scan. Daily QC was performed with a phantom provided by the manufacturer and machine was calibrated, and if the value was within the normal range, then only BMD examination of the study participants was conducted. A coefficient of variation of 2% was noted at both sites over the study period. In FN fracture subjects, BMD measurement was done within 1 week following surgery and uninvolved hip was used for assessment. Hologic machine used National Health and Nutrition Examination Survey (NHANES) (USA-based reference data) to calculate T scores. We also recalculated T scores based on reference data provided by North Indian study, Italian population, and Korean PBMD.,, Overall, we used three databases to recalculate T scores which were done using the following formula:
The categorization of T scores into Normal (+1 to −1), osteopenia (between −1 and −2.5) and osteoporosis (≤−2.5) was as per the world health organization (WHO) guidelines.
The continuous variables were expressed as mean and SD Student's t-test was used to compare the means of continuous variables. BMD categories were expressed as proportions. Agreement between two databases was expressed as kappa. The kappa values were categorised as nil to fair (0–0.40), 0·41–0·65 as moderate, 0·66–0·80 as good, and 0·81–1 as almost perfect agreement. A value of P < 0.05 was considered statistically significant.
| Results|| |
A total of 1956 ambulatory rural South Indian postmenopausal women and 211 FN fracture patients were included in the study. The baseline characteristics of the study participants are shown in [Table 1]. Women with FN fracture were significantly older with lower mean BMI, and lower 25 (OH) vitamin D levels when compared to ambulatory postmenopausal women (P < 0.05). Vitamin D deficiency (<20 ng/ml) was seen in more than half of the study population (52% in ambulatory women and 59% in the fracture group).
The prevalence of osteoporosis at the lumbar spine and FN with manufacturer database (NHANES) was 39% and 22%, respectively. There was a significantly (P < 0.05) greater proportion of participants diagnosed with osteoporosis at lumbar spine (45%) with Italian database and at FN with North Indian database (33%) when compared to other databases [Table 2], [Table 3] and [Figure 1], [Figure 2].
|Table 2: Prevalence of osteoporosis and osteopenia at lumbar spine based on various databases|
Click here to view
|Table 3: Prevalence of osteoporosis and osteopenia at femoral neck based on various databases|
Click here to view
|Figure 1: Categorization of bone mineral density at lumbar spine using different databases|
Click here to view
|Figure 2: Categorization of bone mineral density at femoral neck using different databases|
Click here to view
In subjects with FN fracture, osteoporosis at FN was found in 72% with NHANES, 88% with North Indian, 56% with Italian, and 45% with Korean database [Figure 3]. On comparing manufacturer provided database with the other population-specific reference, there was perfect agreement with North Indian (κ = 0.81 for FN and κ = 0.82 for LS) databases, good agreement with the Italian (κ = 0.78 for FN and κ = 0.74 for LS) and the agreement was found to be lower with the Korean database (κ = 0.6).
|Figure 3: Diagnosis of osteoporosis at femoral neck in fracture subjects with utilization of different databases|
Click here to view
| Discussion|| |
This is the first Indian study to compare the various country based databases (NHANES, Italian, North Indian, and Korean) for the diagnosis of osteoporosis in a large cohort of south Indian rural postmenopausal women. Patients with FN fracture were older with a lower BMI and lower Vitamin D levels when compared to healthy controls. More than half of them had Vitamin D deficiency.
Overall, 40% and 20% had osteoporosis at the lumbar spine and FN, respectively, when Hologic (NHANES) reference database was used. A large number of participants were diagnosed to have osteoporosis at lumbar spine when Italian database was used, and a greater proportion of subjects were diagnosed with osteoporosis at FN when North Indian reference database was used. A perfect agreement was seen between the North Indian database and NHANES database for the diagnosis of osteoporosis at both sites. Most of the participants with FN fracture were diagnosed with having osteoporosis when North Indian database was used.
The prevalence of osteoporosis in this study is similar to earlier reported studies., When considering the population of India >50 years (>100 million), a significant proportion will be diagnosed as having osteoporosis. A higher prevalence of osteoporosis has been reported from the Indian subcontinent when compared to studies from other ethnicities and it has been noted that there were geographic and ethnic disparities in the occurrence of osteoporotic fractures.,,,, Most of the Indian studies used manufacturer Caucasian reference population database (peak bone mass) to calculate T scores. Ethnicity-specific database has been established and used in some countries to diagnose osteoporosis.,,, There have been studies describing the impact of using ethnicity-specific reference on the prevalence of osteoporosis.,
A Swedish study had shown a two-fold increase in prevalence in osteoporosis as per the WHO criteria when Swedish reference database was used due to high peak bone mass in this population as compared to manufacturer provided the database. A decreased prevalence of osteoporosis in women was observed when using a new diagnostic reference data from the Korean population as compared with the reference data provided by the manufacturer of the DXA device. Significant differences in the PBMD at various sites among the Chinese, Japanese, and Caucasian women, which has been shown to influence the prevalence of osteoporosis in a study by Wu et al. Overall, a low PBMD, when compared to Caucasian population, has been reported in Indian studies. Two recent studies found that an Indian population-based database underestimated the diagnosis of osteoporosis., In a study by Shivane et al., PBMD in Indian females was found to be 14%–20% lower than US-based reference standard and height and weight were the significant predictors of bone mass. Patni noted that reference BMD of their study population was 1.5–2 SD lower than the reference Western population.
Marwaha et al. have recently published a normative data for women for Northern India and derived standardized BMD reference data (peak bone mass) which could represent a larger cohort of healthy Indian women. In our study, we used this data to recalculate T scores and most of the FN fracture subjects were correctly identified as having osteoporosis when this North Indian database was used. It should be noted that available reference databases for BMD from India have included a significant proportion of participants with Vitamin D deficiency. 96% of participants in the north Indian database had Vitamin D deficiency. The mean 25-hydroxyv itamin D was 13.6 ± 10 ng/ml in healthy controls of Indian council of medical research reference database.
Osteoporosis and fragility fractures, especially of the hip, are associated with significant morbidity and mortality, especially in postmenopausal women. A mortality of 20% has been reported in participants >60 years. Overall, a study by Dhanwal et al. found an incidence of 159 hip fractures per 100,000 population/year in Indian women >50 years. When considering the number of postmenopausal women in India, a significant proportion of them have osteoporosis which predisposes them to an increased risk of fragility fracture. Thus, the importance of using an appropriate database is vital to correctly identify the individuals at risk. Across all ethnicities, a low BMD is a consistent risk factor for fractures. Ethnicity and race seem to influence the risk of fracture in addition to factors such as age, gender and lifestyle., Although BMD is one of the important factors which will predict fracture, there are other composite indices of bone strength such as weight, height, and differences in hip geometry like longer hip axis length that can influence the bone morphology irrespective of ethnicity impacting the incidence of the fractures., In our study, the impact of using different databases has shown that a variable proportion of FN fracture participants were appropriately identified as having osteoporosis. The implication of using an appropriate database is that it would correctly identify the at risk subjects, who could then be started on anti-osteporotic treatment even before the occurrence of the fracture.
Early diagnosis and treatment with either anti-resorptive or anabolic agent in addition to ensuring adequate calcium and Vitamin D nutrition decreases the incidence of fragility fractures. Hypovitaminosis D is widely prevalent in India in all age groups and it is quite essential that Vitamin D deficiency is treated adequately, especially in postmenopausal women and elderly men with osteoporosis who are candidates for anti-osteoporotic treatment.,, Prospective interventional studies have shown a risk reduction of 40%–50% in fracture incidence after 2–3 years of starting treatment with anti-osteoporotic medications. In the Indian context, treatment of osteoporosis is cost-effective as these drugs are freely available and most of the high-risk population can afford them. Furthermore, such treatment would reduce the morbidity, mortality, and the financial burden associated with osteoporosis and consequent fragility fractures. Finally, it should be borne in mind that ethnicity-specific databases may be influenced by the interplay of many factors such as genetics, level of physical activity, and nutritional status of participants included in the reference population. This also may have an impact on BMD categorization even in south Indian populations, especially when north Indian database is used to define osteoporosis. However, north Indian database is probably ethnically closest available database to the population from south India for categorization of BMD and thus will serve as a useful alternative to Caucasian database till robust reference data from south India is made available. Further longitudinal studies are needed in the Indian context to study the impact of using this Indian database to diagnose and treat osteoporosis on the reduction of incidence of fragility fractures.
| Conclusion|| |
A significant proportion of rural Indian postmenopausal women had osteoporosis. Recently, derived North Indian database correctly identified most of the participants with FN fracture as having osteoporosis and had a perfect agreement with the manufacturer's database. This database could be potentially used in the Indian setting to categorize T-scores and initiate treatment for osteoporosis. Follow-up studies will further validate the impact of utilizing this database in clinical practice to decide the initiation of treatment and its effect in preventing fractures.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Gupta Y, Marwaha RK, Kukreja S, Bhadra K, Narang A, Mani K, et al.
Relationship between BMD and prevalent vertebral fractures in Indian women older than 50 yr. J Clin Densitom 2016;19:141-5.
Jain D, Sidhu GS, Selhi HS, Mears SC, Yamin M, Mahindra P, et al.
Early results of a geriatric hip fracture program in India for femoral neck fracture. Geriatr Orthop Surg Rehabil 2015;6:42-6.
Paul TV, Selvan SA, Asha HS, Thomas N, Venkatesh K, Oommen AT, et al.
Hypovitaminosis D and other risk factors of femoral neck fracture in South Indian postmenopausal women: A pilot study. J Clin Diagn Res 2015;9:OC19-22.
Malhotra N, Mithal A. Osteoporosis in Indians. Indian J Med Res 2008;127:263-8.
] [Full text]
Siris ES, Adler R, Bilezikian J, Bolognese M, Dawson-Hughes B, Favus MJ, et al.
The clinical diagnosis of osteoporosis: A position statement from the national bone health alliance working group. Osteoporos Int 2014;25:1439-43.
El Maghraoui A, Roux C. DXA scanning in clinical practice. QJM 2008;101:605-17.
Leslie WD. Clinical review: Ethnic differences in bone mass – Clinical implications. J Clin Endocrinol Metab 2012;97:4329-40.
Cheung EY, Tan KC, Cheung CL, Kung AW. Osteoporosis in East Asia: Current issues in assessment and management. Osteoporos Sarcopenia 2016;2:118-33.
Lee S, Choi MG, Yu J, Ryu OH, Yoo HJ, Ihm SH, et al.
The effects of the Korean reference value on the prevalence of osteoporosis and the prediction of fracture risk. BMC Musculoskelet Disord 2015;16:69.
Shetty S, Kapoor N, Naik D, Asha HS, Thomas N, Paul TV, et al.
The impact of the Hologic vs. the ICMR database in diagnosis of osteoporosis among South Indian subjects. Clin Endocrinol (Oxf) 2014;81:519-22.
Marwaha RK, Tandon N, Kaur P, Sastry A, Bhadra K, Narang A, et al.
Establishment of age-specified bone mineral density reference range for Indian females using dual-energy X-ray absorptiometry. J Clin Densitom 2012;15:241-9.
Lee KS, Bae SH, Lee SH, Lee J, Lee DR. New reference data on bone mineral density and the prevalence of osteoporosis in Korean adults aged 50 years or older: The Korea National Health and Nutrition Examination Survey 2008-2010. J Korean Med Sci 2014;29:1514-22.
Pedrazzoni M, Girasole G, Bertoldo F, Bianchi G, Cepollaro C, Del Puente A, et al.
Definition of a population-specific DXA reference standard in Italian women: The densitometric Italian normative study (DINS). Osteoporos Int 2003;14:978-82.
Paul TV, Thomas N, Seshadri MS, Oommen R, Jose A, Mahendri NV. Prevalence of osteoporosis in ambulatory postmenopausal women from a semiurban region in Southern India: Relationship to calcium nutrition and Vitamin D status. Endocr Pract 2008;14:665-71.
Thulkar J, Singh S, Sharma S, Thulkar T. Preventable risk factors for osteoporosis in postmenopausal women: Systematic review and meta-analysis. J Midlife Health 2016;7:108-13.
Mithal A, Kaur P. Osteoporosis in Asia: A call to action. Curr Osteoporos Rep 2012;10:245-7.
Cauley JA, Chalhoub D, Kassem AM, Fuleihan Gel H. Geographic and ethnic disparities in osteoporotic fractures. Nat Rev Endocrinol 2014;10:338-51.
Reginster JY, Burlet N. Osteoporosis: A still increasing prevalence. Bone 2006;38:S4-9.
Chen P, Li Z, Hu Y. Prevalence of osteoporosis in China: A meta-analysis and systematic review. BMC Public Health 2016;16:1039.
Ballane G, Cauley JA, Luckey MM, El-Hajj Fuleihan G. Worldwide prevalence and incidence of osteoporotic vertebral fractures. Osteoporos Int 2017;28:1531-42.
Callréus M, McGuigan F, Akesson K. Country-specific young adult dual-energy X-ray absorptiometry reference data are warranted for T-score calculations in women: Data from the peak-25 cohort. J Clin Densitom 2014;17:129-35.
Ribom EL, Ljunggren O, Mallmin H. Use of a swedish T-score reference population for women causes a two-fold increase in the amount of postmenopausal Swedish patients that fulfill the WHO criteria for osteoporosis. J Clin Densitom 2008;11:404-11.
Wu XP, Liao EY, Huang G, Dai RC, Zhang H. A comparison study of the reference curves of bone mineral density at different skeletal sites in native Chinese, Japanese, and American Caucasian women. Calcif Tissue Int 2003;73:122-32.
Singh SK, Patel VH, Gupta B. Comparison of Indian council for medical research and lunar databases for categorization of male bone mineral density. J Clin Densitom 2017. pii: S1094-6950(17) 30037-9.
Shivane VK, Sarathi V, Lila AR, Bandgar T, Joshi SR, Menon PS, et al.
Peak bone mineral density and its determinants in an Asian Indian population. J Clin Densitom 2012;15:152-8.
Patni R. Normal BMD values for Indian females aged 20-80 years. J Midlife Health 2010;1:70-3.
Dhanwal DK, Siwach R, Dixit V, Mithal A, Jameson K, Cooper C. Incidence of hip fracture in Rohtak district, North India. Arch Osteoporos 2013;8:135.
Danielson ME, Beck TJ, Lian Y, Karlamangla AS, Greendale GA, Ruppert K, et al.
Ethnic variability in bone geometry as assessed by hip structure analysis: Findings from the hip strength across the menopausal transition study. J Bone Miner Res 2013;28:771-9.
Nam HS, Kweon SS, Choi JS, Zmuda JM, Leung PC, Lui LY, et al.
Racial/ethnic differences in bone mineral density among older women. J Bone Miner Metab 2013;31:190-8.
El-Kaissi S, Pasco JA, Henry MJ, Panahi S, Nicholson JG, Nicholson GC, et al.
Femoral neck geometry and hip fracture risk: The Geelong osteoporosis study. Osteoporos Int 2005;16:1299-303.
Ishii S, Greendale GA, Cauley JA, Crandall CJ, Huang MH, Danielson ME, et al.
Fracture risk assessment without race/ethnicity information. J Clin Endocrinol Metab 2012;97:3593-602.
Ritu G, Gupta A. Vitamin D deficiency in India: Prevalence, causalities and interventions. Nutrients 2014;6:729-75.
Bruyere O, Reginster JY. Vitamin D status and response to antiosteoporotic therapy. Womens Health (Lond) 2008;4:445-7.
Adami S, Giannini S, Bianchi G, Sinigaglia L, Di Munno O, Fiore CE, et al.
Vitamin D status and response to treatment in post-menopausal osteoporosis. Osteoporos Int 2009;20:239-44.
Qaseem A, Forciea MA, McLean RM, Denberg TD; Clinical Guidelines Committee of the American College of Physicians. Treatment of low bone density or osteoporosis to prevent fractures in men and women: A clinical practice guideline update from the American College of Physicians. Ann Intern Med 2017;166:818-39.
Marwaha RK, Puri S, Tandon N, Dhir S, Agarwal N, Bhadra K, et al.
Effects of sports training & nutrition on bone mineral density in young Indian healthy females. Indian J Med Res 2011;134:307-13.
] [Full text]
[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3]