Indian Journal of Endocrinology and Metabolism

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 22  |  Issue : 6  |  Page : 785--792

Presurgical screening of fine needle aspirates from thyroid nodules for BRAF mutations: A prospective single center experience


Ramamoorthy Hemalatha1, Rekha Pai1, Marie T Manipadam1, Grace Rebekah2, Anish J Cherian3, Deepak T Abraham3, Simon Rajaratnam4, Nihal Thomas4, Pooja Ramakant3, Paul M Jacob3 
1 Department of Pathology, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu, India
2 Department of Biostatistics, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu, India
3 Department of Endocrine Surgery, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu, India
4 Department of Endocrinology and Metabolism, Christian Medical College, Ida Scudder Road, Vellore, Tamil Nadu, India

Correspondence Address:
Paul M Jacob
Department of Endocrine Surgery, Division of Surgery, Christian Medical College, Vellore, Tamil Nadu
India

Objective: Analysis of BRAF V600E mutation in thyroid fine needle aspirates (FNA) is an important adjunct to cytology, particularly among FNA placed in the “indeterminate category.” However, such a prospective evaluation of FNA obtained from patients with thyroid nodules has been lacking from India. Material and Methods: FNA from 277 patients were prospectively evaluated for BRAF mutations by Sanger's sequencing. A subset of 30 samples was also analyzed by pyrosequencing using the PyroMark BRAF mutation kit. Results: Overall, 27.2% of FNA samples were positive for mutations including 19 (35.8%) of the 53 histologically confirmed papillary thyroid carcinoma (PTC), 2 of the 25 follicular variants of PTC, and 1 anaplastic thyroid carcinoma. Only 1 (2.7%) of the 37 samples in the atypia of undetermined significance/follicular lesion of unknown significance category was BRAF positive. The sensitivity of cytology improved marginally from 67.1% to 68.3% when evaluated with BRAF. Further, a comparison of the clinicopathological characteristics of BRAF positive and negative PTCs showed a significant association (P = 0.05) between lymph node metastasis and BRAF positivity. Conclusion: BRAF positivity was lower than that reported from East Asia with the test being useful in confirming malignancies among the suspicious of malignancy and malignant categories.


How to cite this article:
Hemalatha R, Pai R, Manipadam MT, Rebekah G, Cherian AJ, Abraham DT, Rajaratnam S, Thomas N, Ramakant P, Jacob PM. Presurgical screening of fine needle aspirates from thyroid nodules for BRAF mutations: A prospective single center experience.Indian J Endocr Metab 2018;22:785-792


How to cite this URL:
Hemalatha R, Pai R, Manipadam MT, Rebekah G, Cherian AJ, Abraham DT, Rajaratnam S, Thomas N, Ramakant P, Jacob PM. Presurgical screening of fine needle aspirates from thyroid nodules for BRAF mutations: A prospective single center experience. Indian J Endocr Metab [serial online] 2018 [cited 2019 Aug 24 ];22:785-792
Available from: http://www.ijem.in/article.asp?issn=2230-8210;year=2018;volume=22;issue=6;spage=785;epage=792;aulast=Hemalatha;type=0