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  Indian J Med Microbiol
 

Figure 2: Determination of the TCF7L2 rs12255372 SNP incidence in T2DM patients. (a) PCR product that encompasses rs12255372 SNP was electrophoretically separated and visualized in a 1% agarose gel. (b) Allele Specific PCR was carried to detect the G to T transition using allele specific primers; patients presenting T allele amplified a 256bp product. (c) The forward and reverse sequences were aligned and the wild type G, C alleles are presented for a negative patient (d) The forward and reverse sequences for heterozygous positive patient were aligned and the G, A alleles are shown in the aligned sequence

Figure 2: Determination of the TCF7L2 rs12255372 SNP incidence in T2DM patients. (a) PCR product that encompasses rs12255372 SNP was electrophoretically separated and visualized in a 1% agarose gel. (b) Allele Specific PCR was carried to detect the G to T transition using allele specific primers; patients presenting T allele amplified a 256bp product. (c) The forward and reverse sequences were aligned and the wild type G, C alleles are presented for a negative patient (d) The forward and reverse sequences for heterozygous positive patient were aligned and the G, A alleles are shown in the aligned sequence