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The clinical features and urinary hormonal levels were correlated in twenty women with polycystic ovaries. Significantly elevated urinary luteinising hormone (LH) to follicle stimulating hormone (FSH) ratio and estrone glucuronide levels (ElG) were observed in these 20 women (P < 0.001). It was also observed that obesity, hirsutism, amenorrhea and elevated testosterone levels were common (12/20) in group with enlarged ovaries. The study documents the utility of urinary hormone assay with well established reference values in screening women with polycystic ovarian syndrome.