Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Advertise | Login 
 
Search Article 
  
Advanced search 
  Users Online: 394 Home Print this page Email this page Small font sizeDefault font sizeIncrease font size  

 
Table of Contents
BRIEF COMMUNICATION
Year : 2012  |  Volume : 16  |  Issue : 8  |  Page : 512-513

Sertoli cell only syndrome: Status of sertoli cell maturation and function


Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi, India

Date of Web Publication4-Jan-2013

Correspondence Address:
Ashutosh Halder
Department of Reproductive Biology, All India Institute of Medical Sciences, New Delhi - 110 029
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2230-8210.104154

Rights and Permissions
   Abstract 

Background of the study: Mature and functional Sertoli cells are essential for the survival of germ cells in testes. In Sertoli cell only syndrome (SCOS), there is no germ cells. Then, question arises whether absence of germ cells in SCOS secondary to Sertoli cells immaturity or mal function. Sertoli cells maturational and functional status is unclear in SCOS. This study investigated status of maturation and function of Sertoli cells in patients with SCOS. Materials and Methods: The present study was comprised of 37 cases of SCOS and 50 normal control males. Detailed clinical examination and investigation were carried out as per pre-determined proforma. Semen analysis, hormonal analysis (FSH, LH, testosterone, etc.), and fine needle aspiration cytology (FNAC) of testes (bilateral) were performed. Fluorescence in situ hybridization (FISH) with XY probes was carried out in addition to conventional chromosome analysis to find out chromosomal abnormalities, in particular sex chromosome aneuploidy, including mosaicism. Yq microdeletion status was also investigated. The anti-mullerian hormone (AMH), inhibin B, and seminal lactate were estimated by ELISA methods. Results: The study did not find any case of high AMH. About 78% cases had low inhibin B, and 60% had low AMH. FSH was high in about 78% cases. Low level of lactate was found in 49% cases. There was one case of high level of inhibin B. There were 6 (16.2%) cases of chromosomal abnormality (2 mosaic Klinefelter and 4 Klinefelter syndrome) and 4 (10.8%) cases of Yq microdeletion. Conclusion: We conclude that Sertoli cell immaturity does not play any role in SCOS (no case of high AMH). It seems, in majority cases, Sertoli cells are functionally- and/or numerically-deficient (low inhibin B, AMH and lactate). However, in about 22% cases, Sertoli cell function and/or number remains normal (normal inhibin B, AMH). Inhibin B and FSH seems best predictor/marker of Sertoli cell function.

Keywords: Sertoli cell only syndrome, sertoli cells, anti-mullerian hormone, headings not required


How to cite this article:
Jain M, Halder A. Sertoli cell only syndrome: Status of sertoli cell maturation and function. Indian J Endocr Metab 2012;16, Suppl S2:512-3

How to cite this URL:
Jain M, Halder A. Sertoli cell only syndrome: Status of sertoli cell maturation and function. Indian J Endocr Metab [serial online] 2012 [cited 2020 Nov 24];16, Suppl S2:512-3. Available from: https://www.ijem.in/text.asp?2012/16/8/512/104154

SCOS applies to a testis, in which germ cells at any stage are absent, but the tubular architecture is not affected by fibrosis, and supporting cells continue to be present.  Sertoli cells More Details play a central role in development of a functional testis, and hence in the expression of a male phenotype. The Sertoli cells ensure regression of the Mullerian ducts via secretion of AMH. In the male, secretion of AMH by the Sertoli cells commences during embryogenesis and continues throughout life. AMH blood concentration decreases dramatically during puberty [1] and persists at low levels in adults. [2] Sertoli cells also produce inhibin B. [3] Sertoli cells produce lactate at a high rate. [4] To ensure germ cell survival, Sertoli cell must ensure lactate production, even in adverse conditions. Hence, it is important to recognize that absence of germ cells may also be a reflection of underlying abnormalities in the Sertoli cells, such as failure of their maturation. In order to assess the Sertoli cells maturation status and function, it is important to examine the markers such as inhibin B, AMH, and lactate.

Infertile men with idiopathic SCOS were enrolled into the study. Study group comprised of 37 cases of SCOS. Medical history and details of clinical data were collected from the patients. About 5 ml EDTA blood were collected for DNA isolation/interphase FISH and 5 ml for serum to estimate inhibin B, AMH, FSH, lactate besides 1 ml of heparinized blood for cytogenetics. Fifty known fertile subjects were taken as control. An informed consent was obtained from each subject including controls.

Among 37 cases of SCOS, there were 6 cases of chromosomal abnormality (4 cases of Klinefelter syndrome and 2 cases of mosaic Klinefelter syndrome) and 4 cases of Yq microdeletions (2 cases of AZFbc and 1 each of AZFa and AZFc). We have found low level of AMH in 22 cases (59.45%). There was no case with high level of AMH. Similarly, we have found low level of inhibin B and high level of FSH in 29 cases (78.37%). There was one case of high level of inhibin B. Low seminal lactate was noted in 22 cases (59.45%). Table below is showing details of AMH, Inhibin B, Lactate, and FSH in control and SCOS cases.



In this study, we have found a detectable cause in 27% (10/37) of cases (16.2% sex chromosomal abnormality and 10.8% Yq microdeletion), and remaining cases were idiopathic. We have also tried to find out Sertoli cell status through its biomarker AMH. We have found AMH either undetectable (mostly) or normal and no case with high level (expected in Sertoli cell immaturity or hyperplasia or tumor). This suggests that Sertoli cell immaturity or hyperplasia does not exist with SCOS. We hypothesize that Sertoli cells are either dysfunctional or scant in number in most SCOS. We have found inhibin B and FSH equally good predictor/marker of SCOS. We did not find any advantage of adding AMH as another marker over FSH and inhibin B and recommend FSH and inhibin B combination should be used to assess SCOS cases. Seminal lactate as a marker seems complex, and no co-relation was detectable (low, normal as well as high level seen). Furthermore, we did not find any differences in AMH, Inhibin B, and seminal lactate with or without chromosome abnormality or Yq microdeletion.

We concluded that there is an urgent need for more study in SCOS at genomic, epigenomic, and proteomic level to find out underlying etiology. This study should excite more specific work to explore underlying pathophysiology that, in future, could help in management of SCOS cases.

 
   References Top

1.Josso N, Legeai L, Forest MG, Chaussain JL, Brauner R. An enzyme linked immunoassay for anti-Mullerian hormone: New tool for the evaluation of testicular function in infants and children. J Clin Endocrinol Metab 1990;70:23-7.  Back to cited text no. 1
[PUBMED]    
2.Lee MM, Donahoe PK, Hasegawa T, Silverman B, Crist GB, Best S, et al. Mullerian inhibiting substance in human: Normal levels from infancy to adulthood. J Clin Endocrinol Metab 1996;81:571-6.  Back to cited text no. 2
[PUBMED]    
3.Meachem SJ, Nieschlag E, Simoni M. Inhibin B in male reproduction: Pathophysiology and clinical relevance. Eur J Endocrinol 2001;145:561-71.  Back to cited text no. 3
[PUBMED]    
4.Robinson R, Fritz I. Metabolism of glucose by Sertoli cells in culture. Biol Reprod 1981;24:1032-41.  Back to cited text no. 4
    




 

Top
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
    References

 Article Access Statistics
    Viewed1296    
    Printed27    
    Emailed0    
    PDF Downloaded228    
    Comments [Add]    

Recommend this journal