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Year : 2013  |  Volume : 17  |  Issue : 3  |  Page : 529-531

Persistence of histoplasma in adrenals 7 years after antifungal therapy

1 Endocrinology Unit, Dr. RML Hospital, Delhi, India
2 Department of Pathology, Dr. RML Hospital, Delhi, India

Date of Web Publication10-May-2013

Correspondence Address:
Bindu Kulshreshtha
Endocrinology Unit, Dr. Ram Manohar Lohia Hospital, Delhi
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2230-8210.111679

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Adrenal histoplasmosis is an uncommon cause for adrenal insufficiency. The duration of treatment for adrenal histoplasmosis is not clear. Existing treatment regimens advocate antifungals given for periods ranging from 6 months to 2 years. We report here a rare case who showed persistence of histoplasma in adrenal biopsy 7 years after being initially treated with itraconazole for 9 months. This calls for a prolonged therapy with regular review of adrenal morphology and histology in these patients.

Keywords: Addison′s disease, antifungal therapy, histoplasma

How to cite this article:
Kothari D, Chopra S, Bhardwaj M, Ajmani AK, Kulshreshtha B. Persistence of histoplasma in adrenals 7 years after antifungal therapy. Indian J Endocr Metab 2013;17:529-31

How to cite this URL:
Kothari D, Chopra S, Bhardwaj M, Ajmani AK, Kulshreshtha B. Persistence of histoplasma in adrenals 7 years after antifungal therapy. Indian J Endocr Metab [serial online] 2013 [cited 2021 Jun 21];17:529-31. Available from: https://www.ijem.in/text.asp?2013/17/3/529/111679

   Introduction Top

Histoplasmosis is the most common systemic mycosis, characteristically affecting the reticuloendothelial system, blood, and its components. [1],[2] Body's defense mechanisms including both cellular and humoral immunity prevent the affliction by histoplasma. Impaired cellular immunity as in uncontrolled diabetes, AIDS or malignancy could result in invasion and dissemination of the histoplasma.

Adrenals are a common site of invasion by the histoplasma. In overwhelming acute invasion by the histoplasma, the presentation is usually a catastrophic event such as an acute adrenal crisis. [3] However, chronic forms present as subtle features of adrenal insufficiency such as lethargy, hyperpigmentation, weight loss, and gastrointestinal symptoms. Treatment of adrenal histoplasmosis includes antifungals given over a variable period of 6 months to 2 years. However, whether prolonged antifungal treatment can totally eradicate histoplasmosis is unclear. We report here a case whereby the patient diagnosed as adrenal histoplasmosis was treated with 9-month itraconazole therapy and was continued on steroid replacement. There was no regression in the adrenal size and an adrenal biopsy performed 7 years later revealed persistence of histoplasma.

   Case Report Top

A 50 year old male, a diagnosed case of Addison's disease, presented to the Endocrine clinic of this hospital for reevaluation. His clinical history dated 7 years back when he presented with complaints of hyperpigmentation and weight loss for 10 months. There had been no history of tuberculosis in the past. A diagnosis of primary adrenal insufficiency was made (ACTH 743 pg/ml, post-ACTH cortisol 4.8 mcg/dl, PRA 24.7 ng/ml/h (1.3-3.95) and aldosterone 16.6 ng/dl (4-31) and the patient initiated on glucocorticoids and mineralocorticoids. Both the adrenals were enlarged on CT imaging (rt adrena l6.8 × 3.2 cm and Lt adrenal - 5.8 × 4.0 cm). Adrenal biopsy revealed numerous tiny unstained elliptical yeast-like bodies, some of them showing budding. These were Periodic Acid Schiff stain positive [Figure 1]. He received itraconazol 400 mg daily for 9 months which was then stopped and the patient continued on steroid replacement therapy. Hyperpigmentation, appetite, and weight gradually improved while on therapy. He had developed diabetes for the past 2 years and had been receiving oral hypoglycemics. Present evaluation revealed a 75 kg male with no cutaneous striae; blood pressure was 151/93 mm Hg. Investigations revealed a normal hemogram with ESR-40, and normal liver and kidney function tests and Thyroid Function Tests, ACTH 19.5 pg/ml and post-ACTH cortisol levels 4.3 mcg/dl after 1 day of missing the steroid preparation. HIV serology was negative. A repeat CT imaging revealed bilaterally enlarged adrenals with no significant change in either the size or the morphology of the adrenals when compared to the previous CT imaging. Repeat adrenal biopsy revealed necrotic tissue with numerous spores of histoplasma capsulatum that stained positive with the PAS stain and Grocott methanamine silver stain [Figure 2].
Figure 1: Adrenal biopsy shows numerous tiny unstained elliptical yeast-like bodies (staining positive with Periodic Acid - Schiff stain), some of them showing budding (×1000)

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Figure 2: Adrenal biopsy 7 years after initial therapy shows numerous spores of histoplasma capsulatum that stain positive with the PAS stain (×400)

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   Discussion Top

Histoplasmosis is an infectious granulomatous disease caused by dimorphic fungus Histoplasma Capsulatum. [1],[2] Humans get infected by airborne spores of birds or bat's excreta. [1],[2] The fungus has a predilection for the reticuloendothelial system mainly lymph nodes, spleen, and liver. [3] The disease may be classified as acute, chronic, and disseminated. [4] Majority of cases infected with Histoplasma remain asymptomatic. Most symptomatic patients present with variety of self-limited manifestations, the most common being influenza like syndrome and fever. [5] The chronic form of the disease is mainly localized to the lung and manifests with tuberculosis like picture and sequelae. [4] Disseminated histoplasmosis is rare occurring mostly in acute rather than chronic forms; however, the frequency of dissemination in acute histoplasma infections is 1:2000. [6] Most patients who develop acute infection are immunocompromised. An acute rapidly fatal course with diffuse reticuloendothelial involvement characterizes the infection in infants and others who are severely immunosuppressed, while a chronic course with focal organ distribution is more typical in nonimmunocompromised individuals. [6],[7]

Adrenal involvement in histoplasmosis could be either a part of disseminated form in immunosuppressed patients or may occur as a localized adrenal disease. The disseminated forms present as acute adrenal crisis along with multisystem involvement. Godwin et al. reported acute adrenal crisis in 7% of patients presenting with disseminated histoplasmosis. [6] However, subtle or chronic forms of adrenal insufficiency due to focal adrenal involvement may be more common. Sarosi et al. reported around 50% adrenal involvement in patients presenting with various forms of histoplasmosis. [8] CT features of adrenal histoplasmosis vary depending on the stage of the disease. Typically, it includes bilateral adrenal masses with peripheral enhancement and central hypodensities, with calcification seen in the healing phase. [9] These changes are usually bilateral and symmetrical and the shape of adrenal gland is usually preserved.

Treatment of histoplasmosis involves systemic antifungal therapy - ketoconazole or itraconazole for mild to moderate forms and Amphotericin B for severe infections. The duration of therapy is unknown with therapy given for adrenal forms for a period ranging from 6 months to 2 years. [10] Mukherjee et al. reported two immunocompetent patients with histoplasmosis who were treatment with oral itraconazole 200 mg daily for 6 months and 18 months respectively. [11] While the adrenal function was normal 9 years after therapy in the first patient, the second patient continued to have enlarged adrenals 18 months after therapy.

The present case received itraconazole for 9 months. However, there was no reduction in the adrenal size and histoplasma could still be demonstrated in the adrenal biopsy 7 years later. It is unclear whether steroid overdose, development of diabetes or patient's own immune status was responsible for persistence of histoplasma. Whether histoplasma could remain in dormant, latent or subclinical forms for prolonged periods is not known. Desmet et al.[12] reported an immunocompetent patient who developed a reactivation of a latent histoplasma infection, presenting as subacute progressive disseminated histoplasmosis acquired during a previous stay in Africa more than 10 years before. [12] Johnston et al.[13] reported a patient with chronic disseminated histoplasmosis who was followed after successful treatment with amphotericin B for 10 years until his death from chronic obstructive airways disease. Necropsy showed that Histoplasma organisms could still be identified in the adrenal and pancreatic abscesses, though none appeared viable on electron microscopy.

In conclusion, this was a rare case of adrenal histoplasmosis who showed persistence of histoplasma in the adrenal lesions 7 years after itraconazole therapy. This calls for a more aggressive and prolonged therapeutic approach in these patients.

   References Top

1.Adderson E. Histoplasmosis. Pediatr Infect Dis J 2006;25:73-4.  Back to cited text no. 1
2.Wheat LJ. Histoplasmosis: A review for clinicians from non-endemic areas. Mycoses 2006;49:274-82.  Back to cited text no. 2
3.Chedid MF, Chedid AD, Geyer GR, Chedid MB, Severo LC. Histoplasmosis presenting as addisonian crisis in an immunocompetent host. Rev Soc Bras Med Trop 2004;37:60-2.  Back to cited text no. 3
4.Ozols II, Wheat LJ. Erythema nodosum in an epidemic of histoplasmosis in Indianapolis. Arch Dermatol 1981;117:709-12.  Back to cited text no. 4
5.Severo LC, Rizzon CF, Roesch EW, Oliveira Fde M, Porto Nda S. Chronic pulmonary histoplasmosis in Brazil: Report of two cases with cavitation diagnosed by transthoracic needle biopsy. Rev Inst Med Trop Sao Paulo 1997;39:293-7.  Back to cited text no. 5
6.Goodwin RA Jr, Shapiro JL, Thurman GH, Thurman SS, Des Prez RM. Disseminated histoplasmosis: Clinical and pathologic correlations. Medicine (Baltimore) 1980;59:1-33.  Back to cited text no. 6
7.Wheat LJ, Connolly-Stringfield PA, Baker RL, Curfman MF, Eads ME, Israel KS, et al. Disseminated histoplasmosis in the acquired immune deficiency syndrome: Clinical findings, diagnosis and treatment, and review of the literature. Medicine (Baltimore) 1990;69:361-74.  Back to cited text no. 7
8.Sarosi GA, Voth DW, Dahl BA, Doto IL, Tosh FE. Disseminated histoplasmosis: Results of long-term follow-up. A center for disease control cooperative mycoses study. Ann Intern Med 1971;75:511-6.  Back to cited text no. 8
9.Rozenblit AM, Kim A, Tuvia J, Wenig BM. Adrenal histoplasmosis manifested as Addison's disease: Unusual CT features with magnetic resonance imaging correlation. Clin Radiol 2001;56:682-4.  Back to cited text no. 9
10.Subramanian S, Abraham OC, Rupali P, Zachariah A, Mathews MS, Mathai D. Disseminated histoplasmosis. J Assoc Physicians India 2005;53:185-9.  Back to cited text no. 10
11.Mukherjee JJ, Villa ML, Tan L, Lee KO. Bilateral adrenal masses due to histoplasmosis. J Clin Endocrinol Metab 2005;90:6725-6.  Back to cited text no. 11
12.Desmet P, Vogelaers D, Afschrift M. Progressive disseminated histoplasmosis 10 years after return out of Africa in an immunocompetent host. Acta Clin Belg 2004;59:274-8.  Back to cited text no. 12
13.Johnston AW, Brown PA, Ewen SW. Histoplasmosis-A ten year follow-up. J Infect 1996;33:111-3.  Back to cited text no. 13


  [Figure 1], [Figure 2]

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