Home | About us | Editorial board | Search | Ahead of print | Current issue | Archives | Submit article | Instructions | Subscribe | Contacts | Advertise | Login 
Search Article 
Advanced search 
  Users Online: 315 Home Print this page Email this page Small font sizeDefault font sizeIncrease font size  

Table of Contents
Year : 2015  |  Volume : 19  |  Issue : 2  |  Page : 296-299

Seroprevalence of hepatitis C infection in type 2 diabetes mellitus

Department of Medicine, Regional Institute of Medical Sciences, Imphal, Manipur, India

Date of Web Publication14-Jan-2015

Correspondence Address:
Salam Ranabir
Department of Medicine, Regional Institute of Medical Sciences, Imphal - 795 004, Manipur
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2230-8210.149325

Rights and Permissions

Introduction: Hepatitis C is an emerging disease with different studies showing varying prevalence rates across India. In several studies, prevalence of hepatitis C infection was found to be higher in diabetics than nondiabetics. However, none has been reported from India. Objectives: The aim was to determine the sero-prevalence of hepatitis C infection in type 2 diabetes mellitus (T2DM). Settings and Design: Cross-sectional study of all T2DM patients attending endocrine clinic in Regional Institute of Medical Sciences, Imphal from October 2011 to September 2013. Subjects and Methods: All T2DM patients included and exclusion criteria are patients with other forms of diabetes, liver failure, renal failure, malignancy or other chronic illness. Patient's age, sex, height, weight, body mass index, history of risk factors, etc., collected and investigated for blood glucose fasting and prandial levels, transaminases levels, hepatitis C virus (HCV) screening, etc., Statistical Analysis: Statistical analysis was performed using  Statistical Package for the Social Sciences version 20; appropriate test used where applicable. Results: Out of the 192 T2DM patients screened, prevalence rate of HCV sero-positivity is found to be 5.7% (11/192), higher in males. History of jaundice in the past was the only significant history among sero-positive patients. Transaminases levels are significantly higher in sero-postive cases. They had higher fasting and postprandial blood glucose, fasting glucose levels being significantly higher. Conclusion: Our study shows a slightly higher prevalence of hepatitis C infection in type 2 diabetics.

Keywords: Alanine transaminases, aspartate transaminases, hepatitis C virus, type 2 diabetes mellitus

How to cite this article:
Laloo D, Walke P, Bhimo T, Prasad L, Ranabir S. Seroprevalence of hepatitis C infection in type 2 diabetes mellitus. Indian J Endocr Metab 2015;19:296-9

How to cite this URL:
Laloo D, Walke P, Bhimo T, Prasad L, Ranabir S. Seroprevalence of hepatitis C infection in type 2 diabetes mellitus. Indian J Endocr Metab [serial online] 2015 [cited 2021 Sep 27];19:296-9. Available from: https://www.ijem.in/text.asp?2015/19/2/296/149325

   Introduction Top

Hepatitis C virus (HCV) infection is one of the leading causes of chronic liver disease with a global prevalence rate of around 3%. [1] Many studies have shown that HCV-infected patients tend to develop diabetes, [2],[3] reason attributed to insulin resistance. [4] Interestingly, some studies have also shown that the prevalence of HCV infection in diabetics is much higher compared with the normal population. [5],[6],[7] There is no report from India. Hence, the aim of this study was to find out the sero-prevalence rate of HCV infection among type 2 diabetes mellitus (T2DM) patients with a secondary goal to compare the biochemical changes among diabetics with HCV sero-positive and negative.

   Subjects and Methods Top

This cross-sectional study was carried out at a medical institute. All T2DM patients with no history of previous HCV infection attending the Endocrine clinic were included. Anthropometric parameters, duration of diabetes, history of risk factors to HCV infection (jaundice, blood transfusion, high risk sexual behavior, intravenous drug abuse, and surgical procedures) were recorded. Plasma glucose was estimated by the glucose oxidase phenol 4-aminoantipyrine peroxidase kit (enzymatic colorimetric test) with RANDOX auto analyzer manufactured by Randox Laboratories, London, U.K. Alanine transaminases (ALT) and aspartate transaminases (AST) levels were estimated by ultraviolet kinetic inverse spectrophotometric single step method using RANDOX auto analyzer. Glycosylated hemoglobin (HbA1c) was estimated by high-performance liquid chromatography using G8 Analyzer, manufactured by Tosoh Bioscience, Tessenderlo, Belgium. HCV antibody screening was done by rapid immunochromatographic test using Bioline HCV kit manufactured by Bio Standard Diagnostics private Limited, Gurgaon, India. HCV RNA and HCV Genotyping was advised for patients with positive anti-HCV antibody and advised to attend Gastroenterology Clinic, RIMS for further assessment. The data was analyzed using Statistical Package for the Social Sciences software v20 and descriptive statistic such as percentage and proportion and appropriate significance test such as Fisher's exact test, Chi-square test was used for analysis wherever applicable.

   Results Top

A total of 192 T2DM patients were screened, and their characteristics are given in [Table 1]. The prevalence rate of HCV positivity among T2DM patients in the study is found to be 5.7% (11/192). Maximum positivity rate was seen among male patients and all were in the age group of 40-59 years. Among the risk factors for HCV infection, only history of jaundice in the past was significant, however the cause could not be ascertained. Higher number of sero-positives had elevated transaminases compared with sero-negatives [Table 2].
Table 1: Characteristics of the patients in the study (total number (n)=192)

Click here to view
Table 2: Comparisons of hepatitis C positive and negative type 2 DM patients

Click here to view

Sero-positive patients had higher fasting and postprandial blood glucose (fasting blood glucose [FBG] and postprandial glucose [PPBG]) means, of which the FBG levels was significantly higher but the PPBG level was not significantly different. Among the 122 patients who had their HbA1c estimated, no significant difference was found between the sero-positive and sero-negatives. Transaminases levels, were however more significantly elevated in the sero-positive group (P = 0.000) [Table 3].
Table 3: Comparison of the biochemical profi les of HCV serology negative and positive type 2 DM patients

Click here to view

   Discussion Top

The present study found a slightly higher prevalence rate of 5.7% among T2DM when compared to the global prevalence rate of around three percent in the general population. [1] The sero-prevalence in this study is much higher compared to the prevalence of 1.52% among 39,395 apparently healthy blood donors from Manipur. [8]

Simó et al.,[5] study was one of the earliest to demonstrate that prevalence of HCV infection was higher in diabetics than control subjects (11.5% vs. 2.5%; P < 0.001) who are matched for main risk factors. Even though Gray et al.,[6] was the first to show a higher prevalence of HCV infection in T2DM patients (8% in Asian patients), the study was only done on patients with persistently elevated ALT over a period of 3 years. Studies done by Mason et al.,[7] showed a prevalence rate of 4.3% among diabetics. There are also studies much lower low prevalence of HCV infection in diabetics. [9],[10]

The reason for this high prevalence rate is not known, though factors like intravenous drug abuse, multiple sex partners was associated with HCV infection, inference could not be drawn due to low number of patients with these risk factors. History pertaining to risk factors such as intravenous drug usage and multiple sex partners might have been under-reported. History of jaundice in the past was significant for HCV infection but we cannot conclude whether the patient had HCV infection first, before the onset of DM, or after, as these patients have never been screened for HCV infection before. History of surgical procedures and blood transfusions was not significant in our study that goes against other studies that have hypothesized these risks factors as one of the possible cause of acquiring HCV patients in diabetics. [5],[6],[10] Cadranel et al., suggested a nosocomial cause for the increased prevalence of HCV infection in diabetics, with a history of blood transfusion before 1991 and frequent hospital admissions >2 as significant risk factors. [11]

The mean FBG among the diabetics who were sero-positive positive was significantly higher when compared to sero-negatives. Caronia et al.,[12] showed that the fasting insulin levels in patients with HCV-related cirrhosis and diabetes was high and pointed to insulin resistance. As we did not assess the severity of liver damage in these patients who tested positive, conclusion could not be made.

The transaminases level among sero-positives was three times higher than sero-negatives. Though transaminase elevation can be seen among type 2 diabetics, elevation of transaminases by around ≥3 times in diabetics warrants a screening for HCV infection.

Limitation of the study is that Viral RNA was assessed in only five patients; all were positive for HCV RNA; three had genotype 1 and two had genotype 3. Liver histology was also not assessed.

   Conclusion Top

There is a higher prevalence of HCV sero-positivity among patients with type 2 diabetes compared to the general population. It is not certain from this study whether type 2 diabetes predisposes to HCV infection, or the sero-positive cases were already infected and develop diabetes later on. However, it is seen that type 2 diabetics who have more than three times elevation in transaminases have a higher prevalence of HCV sero-positvity.

   References Top

Lavanchy D. The global burden of hepatitis C. Liver Int 2009;29 Suppl 1:74-81.  Back to cited text no. 1
Mehta SH, Brancati FL, Sulkowski MS, Strathdee SA, Szklo M, Thomas DL. Prevalence of type 2 diabetes mellitus among persons with hepatitis C virus infection in the United States. Ann Intern Med 2000;133:592-9.  Back to cited text no. 2
Antonelli A, Ferri C, Fallahi P, Pampana A, Ferrari SM, Goglia F, et al. Hepatitis C virus infection: Evidence for an association with type 2 diabetes. Diabetes Care 2005;28:2548-50.  Back to cited text no. 3
Narita R, Abe S, Kihara Y, Akiyama T, Tabaru A, Otsuki M. Insulin resistance and insulin secretion in chronic hepatitis C virus infection. J Hepatol 2004;41:132-8.  Back to cited text no. 4
Simó R, Hernández C, Genescà J, Jardí R, Mesa J. High prevalence of hepatitis C virus infection in diabetic patients. Diabetes Care 1996;19:998-1000.  Back to cited text no. 5
Gray H, Wreghitt T, Stratton IM, Alexander GJ, Turner RC, O'Rahilly S. High prevalence of hepatitis C infection in Afro-Caribbean patients with type 2 diabetes and abnormal liver function tests. Diabet Med 1995;12:244-9.  Back to cited text no. 6
Mason AL, Lau JY, Hoang N, Qian K, Alexander GJ, Xu L, et al. Association of diabetes mellitus and chronic hepatitis C virus infection. Hepatology 1999;29:328-33.  Back to cited text no. 7
Lalhriatpuii ST, Sharma AB, Singh AM, Singh KR, Devi KM, Pratima K. Hepatitis C virus sero-prevalence among blood donors in a Tertiary Hospital in Manipur. Int J Innov Res Dev 2014;3:190-2.  Back to cited text no. 8
Sjöberg K, Widell A, Verbaan H. Prevalence of hepatitis C in Swedish diabetics is low and comparable to that in health care workers. Eur J Gastroenterol Hepatol 2008;20:135-8.  Back to cited text no. 9
Sotiropoulos A, Peppas TA, Skliros E, Apostolou O, Kotsini V, Pappas SI. Low prevalence of hepatitis C virus infection in Greek diabetic patients. Diabet Med 1999;16:250-2.  Back to cited text no. 10
Cadranel JF, Di Martino V, Lambrey G, Mourlhon C, Nalet B, Anciaux ML, et al. Prevalence of hepatitis C infection and risk factors in hospitalized diabetic patients: Results of a cross-sectional study. Eur J Gastroenterol Hepatol 2008;20:829-36.  Back to cited text no. 11
Caronia S, Taylor K, Pagliaro L, Carr C, Palazzo U, Petrik J, et al. Further evidence for an association between non-insulin-dependent diabetes mellitus and chronic hepatitis C virus infection. Hepatology 1999;30:1059-63.  Back to cited text no. 12


  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Comparison of Frequency Distribution of Diabetes in Hepatitis B and C Patients and Other People in Birjand
Masood Ziaee,Mahboube Saljoughi,Forough Fardinfar,Farah Madarshahian,Azadeh Ebrahimzadeh
Modern Care Journal. 2021; 18(1)
[Pubmed] | [DOI]
2 Seroprevalence and risk factors associated with HBV and HCV infection among subjects with type 2 diabetes from South India
Udyama Juttada,T.P. Smina,Satyavani Kumpatla,Vijay Viswanathan
Diabetes Research and Clinical Practice. 2019; 153: 133
[Pubmed] | [DOI]
3 Hepatitis C virus infections and associated risk factors in patients with diabetes mellitus; case control study in North West Tigray, Ethiopia
Gebretsakan Gebrekristos,Mebrahtu Teweldemedhin,Letebrhan Hagos,Tuom Gebrewahid,Berihu Gidey,Hailay Gebreyesus
BMC Research Notes. 2018; 11(1)
[Pubmed] | [DOI]
4 Prevalence and Genotype Distribution of Hepatitis C Virus Infection among Patients with Type 2 Diabetes Mellitus
Fatemeh Farshadpour,Reza Taherkhani,MohammadReza Ravanbod,SeyedSajjad Eghbali
Medical Principles and Practice. 2018; 27(4): 308
[Pubmed] | [DOI]
5 Effect of Almond Supplementation on Glycemia and Cardiovascular Risk Factors in Asian Indians in North India with Type 2 Diabetes Mellitus: A 24-Week Study
Seema Gulati,Anoop Misra,Ravindra M. Pandey
Metabolic Syndrome and Related Disorders. 2017;
[Pubmed] | [DOI]
6 Infections and diabetes
S. K. Singh,G. R. Sridhar
International Journal of Diabetes in Developing Countries. 2015; 35(2): 59
[Pubmed] | [DOI]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

   Abstract Introduction Subjects and Methods Results Discussion Conclusion Article Tables
  In this article

 Article Access Statistics
    PDF Downloaded324    
    Comments [Add]    
    Cited by others 6    

Recommend this journal